FastQCFastQC Report
Wed 8 May 2024
EGAF00007960501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516404
Total Bases16 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29840.577842154592141No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23220.4496479500546084No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19430.37625579972269774No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG17500.3388819606354715No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17500.3388819606354715No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17150.332104321422762No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15480.2997653000364056No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT15450.2991843595324591No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13190.25542017490182106No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12060.23353808258650202No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11780.22811597121633448No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10520.20371647005058055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10300.19945623968830603No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10280.19906894601900837No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9050.17525038535720097No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8630.16711721830194964No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8480.16421251578221702No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG8420.16305063477432397No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8160.15801581707345413No Hit
CCGGGCGTTCGTCGTGAAATGCAGATGGTGA8020.15530476138837035No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7760.1502699436875005No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7670.14852712217566091No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7230.14000666145111193No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7150.1384574867739212No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7070.13690831209673046No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6980.1351654905848909No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6720.13013067288402103No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6610.1280005577028838No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6460.1250958551831512No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG6360.12315938683666278No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6360.12315938683666278No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6300.12199750582876973No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6190.11986739064763247No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6130.11870550963973941No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6010.11638174762395334No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5980.1158008071200068No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG5790.11212151726167884No Hit
CATCGTTCCCCACTGTGTCGTGATGAAGACG5780.11192787042703001No Hit
GCTCAGGCCATCGACTATGCTGGCTACTACG5580.10805493373405319No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5510.10669940589151129No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5430.10515023121432057No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5330.10321376286783217No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5290.10243917552923681No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5240.1014709413559926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)