FastQCFastQC Report
Wed 8 May 2024
EGAF00007960521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497330
Total Bases15.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT37280.7496028793758671No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19140.38485512637484165No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18090.3637423843323347No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16580.33338025053787224No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA15990.3215169002473207No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG15460.31085999235919815No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG14610.29376872499145434No Hit
GATGGCGCGGACGCTAACGCTCAGGGTAAAG12560.2525486095751312No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT11890.23907666941467434No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11760.23646271087607829No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG11700.2352562684736493No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11560.23244123620131502No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT9960.20026943880320913No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9840.19785655399835123No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9730.19564474292723144No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9550.1920254157199445No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8750.17593951702089156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8690.17473307461846257No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8470.17030945247622303No Hit
CTGGGTGTATCCGCAATCCAGATTGGCAAAT8200.16488046166529266No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8130.16347294552912553No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT7890.15864717591940966No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7530.15140852150483583No Hit
GTATCTGCTATCCAGATCGGTAAGTGGGGCA7210.14497416202521465No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7040.1415559085516659No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7020.1411537610841896No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6880.13833872881185533No Hit
CACGAATCTACCGTGCGTTTCCCGGAGTACA6800.13673013894195002No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA6710.13492047533830653No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6180.12426356745018398No Hit
ATTGTAACCACGGGTTCTACTAAATACTTCA6150.12366034624896949No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6070.1220517563790642No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6040.12144853517784972No Hit
AGCCAGAATACTCAGTCCATCACCAACCTGA6030.12124746144411154No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6000.12064424024289706No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5880.11823135543803914No Hit
GTTCGTTTCCCGGAGTACAACAACAAAGGCC5760.11581847063318118No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5710.11481310196449036No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5660.11380773329579957No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5610.11280236462710876No Hit
ACCCCGCTGCCGCCGGTTTCTCCGGGCGATC5590.11240021715963244No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5390.1083787424848692No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5360.10777552128365471No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5270.10596585768001127No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5160.10375404660889148No Hit
GAACGTTGTTGCGAGGAACTGCAGCGTATGT5020.10093901433655722No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG5020.10093901433655722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)