FastQCFastQC Report
Wed 8 May 2024
EGAF00007960525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496371
Total Bases15.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT35310.711363073185178No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19340.38962791943929037No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16560.3336214242975516No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA16340.32918925561727014No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG16060.32354831366054826No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16030.32294392702232805No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG13890.27983101349595363No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12590.25364092583974485No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12250.2467912106065826No Hit
GATGGCGCGGACGCTAACGCTCAGGGTAAAG12160.24497805069192197No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG12100.24376927741548157No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11200.22563767826887549No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10080.20307391044198797No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10050.20246952380376776No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9880.19904466618718658No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9500.19138910210306403No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9370.18877009333744316No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT8160.164393165595895No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8090.16298293010671452No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8070.1625800056812344No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7900.15915514806465325No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7840.15794637478821286No Hit
GTATCTGCTATCCAGATCGGTAAGTGGGGCA7670.1545215171716317No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7640.1539171305334115No Hit
CTGGGTGTATCCGCAATCCAGATTGGCAAAT7230.14565717981106874No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7120.143441095470928No Hit
CACGAATCTACCGTGCGTTTCCCGGAGTACA7010.14122501113078725No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7000.1410235489180472No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6750.1359869935995455No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6340.12772704287720274No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6190.12470510968610174No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6070.12228756313322092No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5970.12027294100582026No Hit
ATTGTAACCACGGGTTCTACTAAATACTTCA5930.11946709215485997No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5920.11926562994211991No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5770.1162436967510189No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5690.11463199904909835No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5630.11342322577265794No Hit
AGCCAGAATACTCAGTCCATCACCAACCTGA5600.11281883913443774No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5440.10959544373059667No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5400.1087895948796364No Hit
GTTCGTTTCCCGGAGTACAACAACAAAGGCC5360.10798374602867614No Hit
ACCCCGCTGCCGCCGGTTTCTCCGGGCGATC5240.10556619947579532No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5020.10113403079551385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)