FastQCFastQC Report
Wed 8 May 2024
EGAF00007960529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486811
Total Bases15 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA39180.8048298004769818No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC29590.6078334302224067No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT19590.4024149002384909No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG14950.30710070232595404No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG13590.2791637822481415No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC13360.2744391560585114No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13300.2732066448786079No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12940.26581157779918696No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC11300.23212293888182478No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG10930.2245224532724199No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC9490.19494218495473603No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9270.19042297729508986No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG8680.17830328402603884No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA8280.1700865428266822No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC8210.1686486131167948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8060.1655673351670361No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8020.16474566104710042No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA7820.1606372904474221No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA7740.15899394220755075No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7390.15180429365811374No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7240.148723015708355No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7140.14666883040851583No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG7120.146257993348548No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6890.14153336715891793No Hit
ATGATCATCAACCACAACACCAGCGCAATCA6830.14030085597901445No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6600.13557622978938438No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6280.12900283682989908No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6280.12900283682989908No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG6040.1240727921102851No Hit
ACCGCGACTCGCATGACCTCCGGTGAAGCTC5860.12037525857057463No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5830.11975900298062288No Hit
GACGATGTGTGGACCAGCGGTTCTGACTCCG5770.11852649180071938No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5640.11585605091092847No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5630.11565063238094454No Hit
ATGGAATCTTCTGCCAAACGTAAAATGGATC5560.11421270267105714No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5460.11215851737121799No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC5430.11154226178126624No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5310.10907723942145925No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5240.10763930971157185No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5210.1070230541216201No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5170.10620138000168443No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5080.10435261323182919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)