FastQCFastQC Report
Wed 8 May 2024
EGAF00007960533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485719
Total Bases15 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA39480.8128156403187852No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC29170.6005529946326992No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT18800.38705506681846913No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG15700.32323215686435985No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG14600.3005853178483856No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13570.27937964131524606No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC13300.2738208717385978No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12290.25302695591483965No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC11540.23758592931303904No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG10940.2252331080315985No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9650.19867454227650141No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC9330.19208637092639982No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8980.1848805585122262No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG8900.1832335156747008No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8520.17541006219645514No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC8080.16635132659006546No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA8040.16552780517130275No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA7660.1577043516930571No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA7620.1568808302742944No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7190.14802797502259538No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7170.147616214313214No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6950.14308684651001918No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6950.14308684651001918No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6850.14102804296311242No Hit
ATGATCATCAACCACAACACCAGCGCAATCA6520.13423399125832014No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6490.1336163501942481No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG6330.13032226451919732No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6180.12723405919883718No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG6020.12393997352378638No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6020.12393997352378638No Hit
ACCGCGACTCGCATGACCTCCGGTGAAGCTC5710.11755768252837545No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC5370.11055775046889252No Hit
GACGATGTGTGGACCAGCGGTTCTGACTCCG5330.1097342290501298No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5330.1097342290501298No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5260.10829306656729508No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5200.10705778443915104No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5200.10705778443915104No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5160.10623426302038834No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5130.10561662195631631No Hit
ATGGAATCTTCTGCCAAACGTAAAATGGATC5130.10561662195631631No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5070.10438133982817224No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG5040.10376369876410024No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5020.10335193805471887No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4990.10273429699064686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)