FastQCFastQC Report
Wed 8 May 2024
EGAF00007960536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488511
Total Bases21.4 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG25090.5136015360964237No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC21160.43315298939020824No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC20960.4290589157664822No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20560.42087076851903027No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT19190.39282636419650735No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17690.3621208120185625No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17010.3482009616978942No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16430.3363281481890889No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA15730.32199889050604796No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15380.3148342616645275No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG15030.3076696328230071No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA14640.2996861892567414No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA14480.2964109303577606No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG14030.2871992647043772No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA13570.27778289536980744No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT13130.2687759333976103No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11880.24318797324932293No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11860.24277856588695032No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11560.2366374554513614No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG11310.23151986342170391No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG10970.22455993826136977No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10530.2155529762891726No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10480.2145294578832411No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG10240.20961656953476995No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10150.20777423640409323No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10120.20716012536053435No Hit
CGCACCTGCGCCAGCCGGTACGTTAACACCCGCGCCGAAGGTCA9970.20408957014273987No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9930.20327075541799466No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG9230.18894149773495378No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG9170.18771327564783596No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8950.1832097946617374No Hit
AGTCGGGGTCAGAATTGCCGCGCCAGCACCCGCCGGTACGTTAA8700.17809220263207995No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8500.17399812900835396No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT8060.16499116703615682No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7930.16233001918073495No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT7850.16069238973124453No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG7510.1537324645709104No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7400.1514807240778611No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7060.14452079891752692No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6900.14124554001854617No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6630.1357185406265161No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG6500.13305739277109419No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6450.13203387436516273No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT6390.13080565227804491No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC6370.1303962449156723No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6270.12834920810380934No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT6180.12650687497313262No Hit
GTCGGACGGGGTTTTTGCACCACCCATGCCTGCGGAGCCCGGCT6060.12405043079889706No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6060.12405043079889706No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6040.12364102343652444No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5810.11893283876923959No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5760.11790932036330809No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5640.11545287618907252No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT5280.10808354366636576No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5130.10501298844857127No Hit
AGTACCCGGAACGGTCCAGCCACGTTTGACCCATTGACGACCGA5130.10501298844857127No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5050.10337535899908087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)