FastQCFastQC Report
Wed 8 May 2024
EGAF00007960537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488511
Total Bases15.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA45010.9213712690195308No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT32590.6671292969861478No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25410.5201520538943851No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC25410.5201520538943851No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22850.46774791151069267No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22670.4640632452493393No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA21840.4470728397108765No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG19300.3950781046895566No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18020.36887603349771036No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16460.33694225923264776No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15490.31708600215757676No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG15060.30828374386656593No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG14730.30152852238741806No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA14720.3013238187062318No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14150.28965570887861275No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12440.25465137939575566No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11830.24216445484339144No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG11680.23909389962559696No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11590.2372515664949203No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC11500.2354092333642436No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11110.22742578979797795No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10940.22394582721781087No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10680.2186235315069671No Hit
TCTGGTGACGAAGATAGCGACTCCTCCGATG10420.2133012357961233No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT10270.21023068057832886No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10040.20552249591104396No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC9610.19672023762003313No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9270.18976031245969896No Hit
CAGGACCCACGTTTTACTGACACCCTGGTGG8960.1834144983429237No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8880.1817768688934333No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8810.18034394312512922No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT8650.17706868422614844No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC8650.17706868422614844No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8590.17584046213903065No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7810.15987357500649935No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7770.15905476028175414No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7720.15803124187582265No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG7670.15700772346989117No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7650.15659831610751856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7640.15639361242633226No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG7540.1543465756144693No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7530.15414187193328296No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7130.145953724685831No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6900.14124554001854617No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6880.14083613265617353No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA6860.14042672529380096No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA6820.13960791056905575No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC6730.13776557743837908No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6580.1346950222205846No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6520.13346680013346682No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6270.12834920810380934No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6160.12609746761076004No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6130.12548335656720117No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6030.12343631975533817No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5920.12118457926228887No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5900.12077517189991627No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5870.12016106085635737No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5740.1174999130009355No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5700.1166810982761903No Hit
GAATGCGACAACTATCGCCAGGAATACGATA5310.10869765470992465No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA5300.10849295102873834No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5220.10685532157924797No Hit
GAACTGCAGCGTGCGCTGGCAACCCTGCAGT5140.10521769212975757No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4940.1011236185060316No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4930.1009189148248453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)