FastQCFastQC Report
Wed 8 May 2024
EGAF00007960538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467915
Total Bases20.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG24060.5141959543934262No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC20370.43533547759742686No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20110.42977891283673314No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC19850.4242223480760395No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT19280.41204064840836474No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17400.37186241090796407No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16380.35006357992370407No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15610.3336075996708804No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG14660.3133047668914226No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14310.30582477586741186No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA14050.3002682111067181No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG14050.3002682111067181No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13470.287872797409786No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG13360.285521943087954No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT12990.27761452400542835No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12730.27205795924473464No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12160.2598762595770599No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11780.2517551264652768No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11270.2408557109731468No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10910.2331620059198786No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10270.21948430804740177No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG9920.212004317023391No Hit
CGCACCTGCGCCAGCCGGTACGTTAACACCCGCGCCGAAGGTCA9870.21093574687710376No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9830.21008089076007394No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9710.20751632240898454No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG9670.20666146629195475No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG9620.2055928961456675No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9540.20388318391160787No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8780.18764091768804161No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT8740.18678606157101182No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG8720.18635863351249693No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG8700.18593120545398203No Hit
AGTCGGGGTCAGAATTGCCGCGCCAGCACCCGCCGGTACGTTAA8390.17930607054700104No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8390.17930607054700104No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT8130.17374950578630735No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7720.16498723058675188No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7290.1557975273286815No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG7070.15109581868501756No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6700.1431883996024919No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6680.142760971543977No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6610.14126497333917487No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT6610.14126497333917487No Hit
GTCGGACGGGGTTTTTGCACCACCCATGCCTGCGGAGCCCGGCT6130.13100669993481723No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6100.13036555784704487No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG6090.1301518438177874No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5860.12523642114486605No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5790.1237404229400639No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5760.12309928085229155No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5730.12245813876451919No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5710.1220307107060043No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5550.11861128623788508No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5500.11754271609159785No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5480.11711528803308292No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG5240.11198615133090412No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5220.11155872327238922No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5220.11155872327238922No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT4780.1021553059850614No Hit
AGTACCCGGAACGGTCCAGCCACGTTTGACCCATTGACGACCGA4770.10194159195580393No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG4690.10023187972174434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)