FastQCFastQC Report
Wed 8 May 2024
EGAF00007960540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487065
Total Bases21.4 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG25820.5301140504860747No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21380.43895578618870174No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC20780.4266371018241918No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20730.425610544793816No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT19740.4052847155923747No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17430.35785778078901176No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16720.34328067095767506No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16450.33773726299364565No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15740.32316015316230895No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA15150.3110467802038742No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA14730.3024237011487173No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG14580.29934403005758986No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA14520.29811216162113885No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG13880.2849722316323283No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT13550.2781969552318479No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13420.27552790695287077No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12440.25540738915750466No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11590.23795591964111568No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11560.23733998542289017No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11220.2303593976163346No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG10990.2256372352766058No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG10830.22235225277940315No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10800.22173631856117768No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10500.21557697637892273No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10330.2120866824756449No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG10220.20982825700881813No Hit
CGCACCTGCGCCAGCCGGTACGTTAACACCCGCGCCGAAGGTCA10000.20531140607516452No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9930.20387422623263834No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9220.18929711640130167No Hit
AGTCGGGGTCAGAATTGCCGCGCCAGCACCCGCCGGTACGTTAA9020.18519088827979838No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG8930.18334308562512192No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8780.18026341453399444No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG8330.17102440126061202No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG8210.16856066438771006No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG8170.1677394187634094No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT8140.16712348454518391No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT7960.16342787923583094No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7410.1521357519016969No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT6930.142280804410089No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6900.14166487019186352No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6880.14125424737971318No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG6690.13735333066428507No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6650.1365320850399844No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6470.13283647973063142No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC6310.1295514972334288No Hit
GTCGGACGGGGTTTTTGCACCACCCATGCCTGCGGAGCCCGGCT6230.1279090059848275No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6150.1262665147362262No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6040.12400808926939935No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5950.12216028661472288No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5770.11846468130536991No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5560.11415314177779146No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5530.11353720755956596No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5490.11271596193526531No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5470.11230533912311498No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT4970.10203976881935677No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4890.10039727757075545No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG4880.10019196616468029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)