FastQCFastQC Report
Wed 8 May 2024
EGAF00007960541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487065
Total Bases15 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA45860.9415581082607044No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30070.6173713980680197No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC26510.5442805375052611No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25050.514305072218287No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23220.476733084906532No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22130.454354141644339No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA21650.44449919415273115No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG20200.41472904027183227No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18020.36997115374744644No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16190.33239916643569134No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15590.32008048207118145No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG14950.306940552082371No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG14610.29995996427581534No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14450.29667498177861273No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA14380.29523780193608656No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13820.28374036319587737No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC11670.23959841088971698No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11610.238366542453266No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11550.237134674016815No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11480.23569749417428884No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11290.23179657745886073No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG11210.2301540862102594No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10420.21393448513032143No Hit
TCTGGTGACGAAGATAGCGACTCCTCCGATG10330.2120866824756449No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10320.21188137106956978No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT10030.20592734029339002No Hit
CAGGACCCACGTTTTACTGACACCCTGGTGG9260.19011836202560234No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9070.1862174453101742No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA9000.18478026546764806No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8980.1843696426554977No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8870.18211121718867093No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC8820.18108466015829508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8750.17964748031576894No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT8670.17800498906716763No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC8300.17040846704238652No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8190.16815004157555974No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8170.1677394187634094No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG8140.16712348454518391No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG7920.1626066336115303No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7670.15747384845965118No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7380.15151981768347142No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7350.1509038834652459No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7260.14905608081056942No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA7120.14618172112551714No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7080.14536047550121645No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC6900.14166487019186352No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6870.14104893597363802No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6730.1381745762885857No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6680.13714801925820988No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6580.13509490519745823No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA6290.12914087442127847No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6190.12708776036052682No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC6010.12339215505117386No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5990.12298153223902354No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5800.1190806155235954No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5670.11641156724461828No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5660.11620625583854312No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5510.11312658474741565No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5390.11066284787451368No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5290.10860973381376202No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA5250.10778848818946137No Hit
CTGGAACTGCGTGACAACGATAAGACCCGCT5040.10347694866188291No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4950.10162914600720643No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4890.10039727757075545No Hit
GAATGCGACAACTATCGCCAGGAATACGATA4880.10019196616468029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)