FastQCFastQC Report
Wed 8 May 2024
EGAF00007960542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473516
Total Bases20.8 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG25040.5288100085319187No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21090.44539149680264234No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21030.44412438016878No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC19750.41709255864638156No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT18690.3947068314481454No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16510.3486682604178106No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15740.3324069302832428No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15670.33092862754373664No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15020.3172015306768937No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14610.30854290034550047No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA14060.2969276645350949No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG13750.29038089526013905No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG13370.28235582324567704No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT13310.28108870661181456No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13160.27792091502715854No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12390.2616595848925908No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12080.2551128156176349No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11790.24898841855396653No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11750.24814367413139152No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG11160.23568369389841104No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10430.2202671081864182No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10280.21709931660176215No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10210.21562101386225596No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG10210.21562101386225596No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10070.21266440838324366No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9820.2073847557421502No Hit
CGCACCTGCGCCAGCCGGTACGTTAACACCCGCGCCGAAGGTCA9230.1949247755091697No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG9220.19471358940352596No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8620.18204242306490173No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG8590.1814088647479705No Hit
AGTCGGGGTCAGAATTGCCGCGCCAGCACCCGCCGGTACGTTAA8480.17908581758588937No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT8470.17887463148024565No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG8450.1784522592689582No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8150.17211667609964607No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7930.16747058177548382No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT7820.1651475346134027No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG7670.16197974302874665No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7340.15501060154250332No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6920.14614078510546635No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT6840.14445129626031644No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6830.1442401101546727No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG6780.14318417962645402No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6430.13579266592892322No Hit
GTCGGACGGGGTTTTTGCACCACCCATGCCTGCGGAGCCCGGCT6160.13009064107654228No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5960.12586691896366756No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5910.12481098843544886No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5850.12354387180158644No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5770.12185438295643652No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5580.11784184694920552No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5530.11678591642098685No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5510.11636354420969934No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT5450.11509642757583692No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5380.11361812483633078No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG5280.1115062637798934No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5260.11108389156860592No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5260.11108389156860592No Hit
AGTACCCGGAACGGTCCAGCCACGTTTGACCCATTGACGACCGA5240.11066151935731844No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG4750.10031340018077531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)