FastQCFastQC Report
Wed 8 May 2024
EGAF00007960543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473516
Total Bases14.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA44190.933231400839676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39420.8324956284476132No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT31920.6741060492148101No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC24690.5214184948343878No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24090.5087473284957635No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21980.464187060204935No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20620.4354657498373867No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA20200.42659593340034974No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG18650.39386208702557035No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17000.35901637959435373No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15700.3315621858606679No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG14400.304107992126982No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13960.29481580347865755No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA13790.291225639682714No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13580.28679073146419554No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG13360.2821446371400333No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11960.25257858234991004No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC11560.24413113812416054No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11540.24370876591287305No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11410.24096334653950446No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10810.2282921802008802No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10600.22385727198236172No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10480.22132303871463688No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG10380.2192111776581995No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9880.20865187237601263No Hit
TCTGGTGACGAAGATAGCGACTCCTCCGATG9470.1999932420446194No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9090.19196817003015737No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT9010.1902786811850075No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC8790.18563258686084524No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8540.1803529342197518No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA8540.1803529342197518No Hit
CAGGACCCACGTTTTACTGACACCCTGGTGG8500.17950818979717686No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8440.17824107316331445No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG8040.16979362893756494No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7900.1668370234585526No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7800.16472516240211524No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC7750.16366923187389654No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT7680.16219092913439037No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7490.15817839312715937No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7440.1571224625989407No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7390.156066532070722No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7380.15585534596507825No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG7290.15395467101428464No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7270.15353229880299715No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA7080.14951976279576612No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6740.14233943520387907No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC6560.1385380853022918No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6390.13494792150634827No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6140.12966826886525482No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6040.12755640780881744No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5950.1256557328580238No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5910.12481098843544886No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5860.12375505790723017No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5760.12164319685079279No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5540.11699710252663056No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5530.11678591642098685No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5510.11636354420969934No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5470.1155187997871244No Hit
GAATGCGACAACTATCGCCAGGAATACGATA5360.11319575262504328No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5320.11235100820246834No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5290.11171744988553714No Hit
GAACTGCAGCGTGCGCTGGCAACCCTGCAGT5240.11066151935731844No Hit
CTGGAACTGCGTGACAACGATAAGACCCGCT4880.1030588195541439No Hit

[OK]Adapter Content

Adapter graph