FastQCFastQC Report
Wed 8 May 2024
EGAF00007960544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519011
Total Bases22.8 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG18910.3643468057517085No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18840.362998086745753No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG18400.35452042442260373No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG17610.3392991670696767No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13840.26666101489178456No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG13240.2551005662693083No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13180.25394452140706075No Hit
GTCCAGACGCGCCAGTGCCAGCAGCATCTGGTAGGCCTGTTCGT11600.22350200670120673No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG11460.22080456868929563No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10810.2082807493482797No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG10510.2025005250370416No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10180.19614227829467967No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG9950.19171077298939715No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT9370.18053567265433682No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9080.17494812248680663No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8780.16916789817556852No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8630.16627778601994947No Hit
ATCCAGGGAATCGGTACCGTTCTGCTGAGCACGCTGTTCTGCGT8370.16126825828354313No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT8050.15510268568488914No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG8000.15413931496634944No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7530.14508363021207643No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7500.14450560778095262No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7480.14412025949353674No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7350.14161549562533357No Hit
CGGGAAAGACAGATCTTCGTCAGAAGAAGAGCTGGATGCCGCAG7340.14142282148162563No Hit
GGACTGGCCACGGTGGCCACCATCGTAGCGTTCCCAGCCGCTCG7130.13737666446375896No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7080.13641329374521927No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6910.13313783330218434No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6820.13140376600881293No Hit
CAGGACCGCTTTGTCAGCGTCGTTTACGCCCAGGGTACCGCCAG6310.12157738467970813No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6280.12099936224858432No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6180.11907262081150495No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6100.11753122766184146No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC6090.11733855351813353No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6090.11733855351813353No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5530.10654880147048906No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT5500.10597077903936525No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5490.10577810489565732No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5470.10539275660824145No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5310.10230997030891446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)