FastQCFastQC Report
Wed 8 May 2024
EGAF00007960545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519011
Total Bases16 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20190.38900909614632445No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG19360.3730171422185657No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18670.35972262630271806No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG18440.3552911209974355No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA18280.3522083346981085No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14550.28034087909504807No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12770.2460448815150353No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG12470.24026465720379722No Hit
GCAGATGGTACTACCGTTACGAGCGGCTCCA11610.22369468084491467No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11360.21887782725221624No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11330.21829980482109243No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11230.21637306338401305No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10950.2109781873601908No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA10860.2092441200668194No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG10720.20654668205490828No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9890.19055472812714952No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9860.1899767056960257No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA9740.18766461597153047No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9540.18381113309737174No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9140.17610416734905426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9140.17610416734905426No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8770.1689752240318606No Hit
TCCGTCTATAACTCTGGCCGTAAAGGTCCGG8350.16088290999612725No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA8220.15837814612792409No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8040.15491001154118123No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7960.15336861839151772No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7840.15105652866702246No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7750.14932246137365104No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC7680.1479737423676955No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7090.1366059678889272No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7060.1360279454578034No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6990.13467922645184785No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA6960.13410120402072404No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6930.13352318158960022No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6580.12677958655982244No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6550.12620156412869862No Hit
GTTGCAGGTGGCGCAGATATCGATTACGCAA6410.1235041261167875No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC6380.1229261036856637No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6340.12215540711083195No Hit
GCCCCACCGTATACCAACGAACAGGCATACC6220.11984331738633672No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA6070.11695320523071766No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5980.11521913793734623No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5740.11059495848835574No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5580.10751217218902874No Hit
GCAGCTCACTCCCGTTCCGGCTCCGTGAGCC5510.10616345318307319No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5430.1046220600334097No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5350.1030806668837462No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5340.10288799274003828No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5330.10269531859633033No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG5240.10096125130295888No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5230.10076857715925096No Hit

[OK]Adapter Content

Adapter graph