FastQCFastQC Report
Wed 31 May 2023
EGAF00007960546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497663
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG18630.37434971054709715No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG18120.3641018118686742No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18080.3632980551095822No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16590.33335811583340536No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14140.2841280143390206No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG12890.2590106156173957No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12550.25217868316511377No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10960.22022935199120688No Hit
GTCCAGACGCGCCAGTGCCAGCAGCATCTGGTAGGCCTGTTCGT10690.21480399386733592No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG10680.21460305467756294No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10060.202144824911637No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10010.201140128962772No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9270.18627062891957008No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG9050.1818499667445641No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT8720.17521897348205515No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG8400.16878891940931914No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8270.16617670994227016No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8170.16416731804454018No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7900.15874195992066922No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7470.15010157476043026No Hit
ATCCAGGGAATCGGTACCGTTCTGCTGAGCACGCTGTTCTGCGT7460.14990063557065725No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7460.14990063557065725No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7310.14688654772406226No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7230.1452790342058783No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7220.14507809501610527No Hit
GGACTGGCCACGGTGGCCACCATCGTAGCGTTCCCAGCCGCTCG6970.1400546152717803No Hit
CGGGAAAGACAGATCTTCGTCAGAAGAAGAGCTGGATGCCGCAG6870.1380452233740503No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6450.12960577740358434No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6420.12900295983426535No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6190.12438135846948638No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6180.12418041927971338No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5980.1201616354842534No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5960.1197597571047074No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC5960.1197597571047074No Hit
CAGGACCGCTTTGTCAGCGTCGTTTACGCCCAGGGTACCGCCAG5960.1197597571047074No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5840.11734848682743143No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5600.11252594627287943No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT5550.11152125032401444No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5420.10890904085696544No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5400.10850716247741946No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5350.10750246652855447No Hit
TTCTTCGTCAGAGTCCTTCAGGTGACGGTAACCGTTTTTGCGGT5180.10408650030241347No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5020.10087147326604551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)