FastQCFastQC Report
Wed 8 May 2024
EGAF00007960548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517552
Total Bases22.7 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG19040.3678857390175287No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG18800.36324852381982875No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18270.353008006924908No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG17600.340062447831329No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13930.26915169876650075No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13470.2602637029709092No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG13230.2556264877732093No Hit
GTCCAGACGCGCCAGTGCCAGCAGCATCTGGTAGGCCTGTTCGT12060.23302006368442202No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG11440.22104059109036384No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG10750.20770859739697653No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10690.20654929359755153No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10250.19804773240176834No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9780.1889665193062726No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG9490.18336321760905183No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT8920.17234983151451447No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8890.171770179614802No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG8860.1711905277150895No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8780.1696447893158562No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7880.1522552323244814No Hit
ATCCAGGGAATCGGTACCGTTCTGCTGAGCACGCTGTTCTGCGT7830.15128914582496059No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7690.14858410362630228No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7520.1452994095279315No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7490.144719757628219No Hit
GGACTGGCCACGGTGGCCACCATCGTAGCGTTCCCAGCCGCTCG7470.14433332302841068No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7390.14278758462917734No Hit
CGGGAAAGACAGATCTTCGTCAGAAGAAGAGCTGGATGCCGCAG7270.14046897703032737No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7060.13641141373233995No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7020.13563854453272328No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6910.13351315423377746No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6790.1311945466349275No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6500.12559124493770674No Hit
CAGGACCGCTTTGTCAGCGTCGTTTACGCCCAGGGTACCGCCAG6280.12134046433981513No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC6250.12076081244010263No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6160.11902185674096516No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6140.11863542214115683No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5990.11573716264259436No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5960.11515751074288189No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5870.11341855504374439No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT5690.10994064364546945No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5660.10936099174575695No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5310.1025983862491112No Hit
GTTACGTTCGAAACGAATCAGATCGGTACCCTGCGCCATAGAGC5240.10124586514978205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)