FastQCFastQC Report
Wed 8 May 2024
EGAF00007960549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517552
Total Bases16 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG19770.3819906019105327No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19710.38083129811110766No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG18800.36324852381982875No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA18410.3557130491235663No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18030.3483707917272081No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14600.2820972578600798No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12990.25098927257550935No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG12650.24441988437876772No Hit
GCAGATGGTACTACCGTTACGAGCGGCTCCA12000.23186075988499708No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11490.2220066775898847No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA11090.2142779855937181No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11040.2133118990941973No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10850.20964077039601817No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10820.2090611184963057No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG10630.2053899897981266No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10430.20152564380004329No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10220.19746808050205583No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9960.19244443070454756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9280.1793056543110644No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA9270.17911243701116022No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9150.17679382941231025No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA9070.17524809101307695No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9010.17408878721365198No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8060.15573314372275635No Hit
TCCGTCTATAACTCTGGCCGTAAAGGTCCGG7970.1539941880236189No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7960.15380097072371474No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7920.15302810152409807No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7910.15283488422419392No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC7590.14665193062726065No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7500.14491297492812316No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7360.14220793272946486No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA7330.14162828082975237No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7070.1366046310322441No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7010.13544532723281913No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6890.13312671963396913No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6870.13274028503416083No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6480.1252048103378984No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA6410.12385228923856927No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC6400.1236590719386651No Hit
GCCCCACCGTATACCAACGAACAGGCATACC6280.12134046433981513No Hit
GTTGCAGGTGGCGCAGATATCGATTACGCAA6270.12114724703991098No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6040.11670324914211519No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5930.11457785884316939No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5930.11457785884316939No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5810.11225925124431942No Hit
GCAGCTCACTCCCGTTCCGGCTCCGTGAGCC5530.10684916684700282No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5390.10414412464834451No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5200.10047299595016539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)