FastQCFastQC Report
Wed 8 May 2024
EGAF00007960550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504513
Total Bases22.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG18560.36787951945737773No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG18350.3637170895497242No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG17200.3409228305316216No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17060.33814787725985257No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13900.27551321769706627No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG12940.2564849666906502No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12280.24340304412373914No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG11350.22496942596127353No Hit
GTCCAGACGCGCCAGTGCCAGCAGCATCTGGTAGGCCTGTTCGT11020.21842846467781799No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG10620.210500026758478No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10350.20514833116292344No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10300.20415727642300596No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9410.18651650205247436No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG9410.18651650205247436No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT9330.18493081446860635No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG8620.1708578371617778No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8550.1694703605258933No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8390.16629898535815726No Hit
ATCCAGGGAATCGGTACCGTTCTGCTGAGCACGCTGTTCTGCGT7940.15737949269889973No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7810.1548027503751142No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7510.1488564219356092No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7400.14667610150779067No Hit
CGGGAAAGACAGATCTTCGTCAGAAGAAGAGCTGGATGCCGCAG7310.14489220297593916No Hit
GGACTGGCCACGGTGGCCACCATCGTAGCGTTCCCAGCCGCTCG7270.14409935918400518No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7190.14251367160013717No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6930.13736018695256613No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6800.1347834446287806No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6680.1324049132529786No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6630.13141385851306112No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6540.1296299599812096No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC6270.12427826438565508No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6170.12229615490582006No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6060.12011583447800156No Hit
CAGGACCGCTTTGTCAGCGTCGTTTACGCCCAGGGTACCGCCAG5930.11753909215421604No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT5840.11575519362236454No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5660.11218739655866153No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5560.11020528707882651No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5530.109610654234876No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5500.1090160213909255No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5490.10881781044294199No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5100.10108758347158546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)