FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007960563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517038
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43000.8316603421798784No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24870.4810091327910135No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23470.4539318193246918No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG23040.44561521590289305No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC19850.3839176230760602No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG19470.3765680665637729No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG18530.3583875846649569No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16210.31351660806362397No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15850.30655387031514125No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG15190.29378885110958963No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG15090.29185475729056665No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC14380.27812269117550353No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG13840.2676785845527795No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC12250.2369264928303142No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12110.23421876148368206No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11930.2307373926094407No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11150.2156514608210615No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10930.21139645441921096No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10150.19631052263083176No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG9930.19205551622898123No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9880.19108846931946974No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9680.18722028168142382No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9630.1862532347719123No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9600.18567300662620542No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9600.18567300662620542No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9500.18373891280718244No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9490.18354550342528014No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8590.16613865905407338No Hit
CTGGACGCTACCCGCGAGTCTCTGGATGTTA8460.1636243370893435No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG8370.16188365265222285No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8110.15685500872276312No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7950.15376045861232635No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7910.15298682108471717No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7650.14795817715525744No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7580.14660431148194136No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7580.14660431148194136No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG7150.13828770806014257No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6660.12881064834693No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6650.12861723896502772No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG6330.1224281387441542No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6220.12030063554322892No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6070.11739949481469447No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6050.11701267605088987No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5710.11043675706621175No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5600.1083092538652865No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5590.10811584448338421No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5570.1077290257195796No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5560.1075356163376773No Hit
ATCGCTGATCTGATCGCACGTGGTGACGACA5510.10656856942816582No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5480.10598834128245893No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC5400.10444106622724054No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5400.10444106622724054No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5350.10347401931772907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)