FastQCFastQC Report
Wed 8 May 2024
EGAF00007960577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525671
Total Bases16.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA69161.3156518050263377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41490.7892769431830936No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35020.6661961569118328No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA32070.6100774058298822No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25570.486425920395076No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20480.389597295646897No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20040.3812270412482332No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17850.3395660023094293No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17710.3369027395462181No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16780.319211065476315No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15400.2929589039532331No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14940.2842081834455391No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14280.2716528018475434No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13610.25890718719503264No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13100.24920530141476324No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12070.22961129679970935No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11240.21382195327495718No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA9230.17558510931742477No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9160.17425347793581916No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9140.173873011826789No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8780.1670246218642459No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7980.15180597750303898No Hit
AAGCTGAAGGAAGCGGAAGCACGTGCCGAGT7940.15104504528497864No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7710.14666968503113165No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7590.1443868883769506No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6980.13278267205153033No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6950.13221197288798506No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC6950.13221197288798506No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6900.13126080761540962No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6690.12726591347059282No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6510.12384171848932127No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6360.12098822267159497No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC6250.11889565907192902No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6160.11718356158129326No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5820.1107156377277803No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5590.10634027747393332No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5580.10615004441941822No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5570.10595981136490315No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5520.10500864609232771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)