FastQCFastQC Report
Wed 8 May 2024
EGAF00007960579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522630
Total Bases16.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT66671.27566347128944No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC41510.7942521477909803No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37060.7091058683963799No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25320.4844727627575914No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23910.45749382928649335No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22100.4228612976675659No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21140.4044926621127758No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG19170.3667986912347167No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC19000.3635459120218893No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18790.35952777299427896No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG18400.3520655148001454No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17960.34364655683753326No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16710.3197290626255669No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14700.28126973193272486No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14050.26883263494250237No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13940.2667278954518493No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12940.2475939000822762No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC12920.2472112201748847No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12780.24453246082314448No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12220.23381742341618356No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12110.23171268392553052No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11150.2133440483707403No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10890.2083692095746513No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10710.20492509040812812No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10520.20128963128790922No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10360.19822819202877756No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10140.19401871304747145No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9800.18751315462181659No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9500.18177295601094465No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9490.18158161605724893No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9370.17928553661290014No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8860.16952719897441784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8800.16837915925224345No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8500.16263896064137154No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8460.1618736008265886No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8370.16015154124332703No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7640.14618372462353862No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7530.14407898513288558No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7290.13948682624418807No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6890.1318332280963588No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6840.13087652832788013No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6800.1301111685130972No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6720.12858044888353137No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6630.12685838930026977No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6620.12666704934657405No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6590.12609302948548687No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6590.12609302948548687No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6510.12456230985592102No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC6480.12398828999483381No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6340.12130953064309359No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6340.12130953064309359No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6280.1201614909209192No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6240.11939613110613628No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6100.11671737175439603No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5970.11422995235635153No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5830.11155119300461129No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA5790.11078583318982836No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5740.10982913342134971No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5710.10925511356026252No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5690.10887243365287107No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5670.1084897537454796No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5460.10447161471786924No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5350.1023668752272162No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5290.10121883550504181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)