FastQCFastQC Report
Wed 8 May 2024
EGAF00007960581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498650
Total Bases15.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62881.2610047127243558No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40860.8194124135164946No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39550.7931414820014037No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG34990.7016945753534543No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22480.45081720645743506No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22390.44901233329990975No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20180.4046926702095659No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19640.39386343126441387No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG18300.3669908753634814No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17980.3605735485811692No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16930.3395166950767071No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16870.3383134463050236No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC16580.3324977439085531No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15030.30141381730672817No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13640.27353855409605937No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC13330.26732176877569436No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13300.2667201443898526No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13120.26311039807480197No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12380.2482703298907049No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11160.22380427153313948No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11160.22380427153313948No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10850.2175874862127745No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10090.20234633510478295No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9970.1999398375614158No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9960.19973929609946856No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9930.19913767171362678No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9750.19552792539857616No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9530.1911160132357365No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9310.1867041010728968No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9240.18530031083926604No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9150.1834954376817407No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8230.165045623182593No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8090.16223804271533138No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7960.15963100371001704No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7960.15963100371001704No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7730.1550185500852301No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7670.1538153013135466No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7610.15261205254186302No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7090.14218389652060562No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6790.13616765266218792No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6790.13616765266218792No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6720.13476386242855712No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6360.12754436979845585No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6330.12694274541261405No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6260.12553895517898325No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC6200.12433570640729971No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6150.12333299909756343No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6150.12333299909756343No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA6060.1215281259400381No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5930.11892108693472374No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5810.11651458939135667No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5800.1163140479294094No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5760.11551188208162037No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5620.11270430161435877No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5590.11210267722851698No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5550.11130051138072795No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5460.10949563822320264No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5450.1092950967612554No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5340.10708914067983556No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5260.1054848089842575No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5250.10528426752231022No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5030.10087235535947056No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG4990.10007018951168153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)