FastQCFastQC Report
Wed 8 May 2024
EGAF00007960582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521010
Total Bases22.9 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT35550.6823285541544308No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA33130.6358803093990518No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC31470.60401911671561No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28990.5564192625861308No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA23160.44452121840271774No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA20300.3896278382372699No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20240.3884762288631696No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19170.36793919502504746No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG17950.3445231377516746No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG17640.3385731559854897No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT17420.3343505882804553No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT16280.31247001017254944No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC16060.308247442467515No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG14350.27542657530565634No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA14280.2740830310358726No Hit
ACCCAGCGGGATGGCCGGCAGAGAATAGCCATCCGGAAAAACGG13910.2669814398955874No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13710.2631427419819197No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG12730.24433312220494807No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG11930.22897833055027733No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11310.21707836701790753No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT11060.21227999462582292No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT11050.21208805973013953No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG10190.1955816587013685No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9470.18176234621216483No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG9020.17312527590641255No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC9010.17293334101072916No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8780.16851883841001133No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG8660.16621561966181073No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8560.16429627070497688No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8490.16295272643519318No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8390.16103337747835933No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8280.15892209362584211No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG7910.1518205024855569No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7770.14913341394598953No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG7640.14663826030210553No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG7440.14279956238843786No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7210.13838505978772No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7180.13780925510066985No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7030.1349302316654191No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG6620.12706090094240036No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6540.12552542177693327No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG6540.12552542177693327No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6460.12398994261146618No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6350.12187865875894896No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6340.1216867238632656No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6310.12111091917621543No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG6230.11957544001074837No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6050.11612061188844744No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6030.11573674209708067No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5970.11458513272298036No Hit
GGCACCAACGAACGCACGCACAGCGGATGCGGTTACGTCCTGCG5860.11247384887046313No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5800.11132223949636283No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5730.10997869522657913No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5560.10671580199996163No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5540.10633193220859485No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5530.10613999731291146No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5510.10575612752154469No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5460.10479645304312776No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC5350.10268516919061053No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5330.10230129939924379No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5300.10172549471219362No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG5250.1007658202337767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)