FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007960583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521010
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62911.20746242874417No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42210.8101571946795647No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37530.7203316634997409No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25000.4798372392084605No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22750.4366518876796991No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21850.4193777470681945No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20790.3990326481257557No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG18930.3633327575286463No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18910.3629488877372795No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18780.36045373409339554No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC18110.34759409608260877No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17450.3349263929675054No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15870.30460067944953073No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14340.275234640409973No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13990.2685169190610545No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13980.2683249841653711No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC13490.2589201742768853No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12680.2433734477265312No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12680.2433734477265312No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12050.23128154929847794No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11810.22667511180207672No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11180.21458321337402353No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10700.20537033838122107No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10640.2042187290071208No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10610.20364292432007064No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10320.1980768123452525No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10310.19788487744956912No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9910.19020748162223375No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9830.18867200245676666No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9740.1869445883956162No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8970.1721656014279956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8950.17178173163662885No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8660.16621561966181073No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8480.16276079153950979No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8270.15873015873015872No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8220.1577704842517418No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7970.1529721118596572No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7680.14740599988483907No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7570.14529471603232186No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7520.14433504155390492No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7140.1370415155179363No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7120.13665764572656955No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC6960.1335866873956354No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6860.13166733843880155No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6730.12917218479491757No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6620.12706090094240036No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6570.12610122646398342No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6460.12398994261146618No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6420.12322220302873266No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6340.1216867238632656No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6330.1214947889675822No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6320.12130285407189881No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6080.11669641657549759No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6000.11516093741003053No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA6000.11516093741003053No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5930.11381739314024683No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5840.11208997907909636No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5820.1117061092877296No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5770.11074643480931269No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5710.10959482543521237No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5660.10863515095679546No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5640.10825128116542869No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5430.10422064835607762No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5420.10402871346039423No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5390.1034529087733441No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5280.10134162492082686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)