FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007960584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506394
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT33470.660947799539489No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA31120.614541246539256No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC28920.5710968139432931No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28160.5560887372283242No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA22260.43957866799369655No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20200.39889888110838595No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA19940.39376453907431763No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18890.3730296962444263No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG17410.34380344158896037No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG17050.33669435261871195No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT17030.3362994032314759No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC15590.30786304735048203No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15440.30490092694621185No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA14560.2875231539078267No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG13900.2744898241290379No Hit
ACCCAGCGGGATGGCCGGCAGAGAATAGCCATCCGGAAAAACGG13020.2571120510906527No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12620.24921306334593224No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11870.23440246132458128No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11220.2215666062394104No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG11140.21998680869046633No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10590.20912570054147558No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG10530.20794085237976753No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9860.19471004790736068No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9540.18839085771158426No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC9160.18088681935409975No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG9040.17851712303068362No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8710.1720004581412892No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8500.16785348957531093No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8210.16212672346038856No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG8030.15857217897526432No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC8020.1583747042816463No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG7850.15501763449014008No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG7750.15304288755395995No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7600.15008076714968976No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG7460.14731612143903758No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7360.14534137450285747No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7140.14099693124326118No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6920.1366524879836649No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6710.13250551941768662No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6610.1305307724815065No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6580.12993834840065246No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6500.12835855085170836No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6460.1275686520772363No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6270.12381663289849407No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6240.12322420881764001No Hit
GGCACCAACGAACGCACGCACAGCGGATGCGGTTACGTCCTGCG6140.1212494618814599No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT5990.11828734147718971No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG5970.11789239208995367No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG5950.11749744270271766No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5890.11631259454100958No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5710.11275805005588535No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5650.11157320189417726No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5570.10999340434523316No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5490.10841360679628906No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5330.10525401169840085No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5290.10446411292392879No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG5270.10406916353669278No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG5260.10387168884307477No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5250.10367421414945674No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5230.10327926476222073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)