FastQCFastQC Report
Wed 8 May 2024
EGAF00007960585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506394
Total Bases15.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT67341.3297945868236987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41380.8171502821913371No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39770.7853568565188371No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG34080.6729937558501877No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23340.460905934904442No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22020.4348392753468643No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20700.40877261578928664No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20000.3949493872360257No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19970.39435696315517166No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18200.3594039423847834No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17760.3507150558655908No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC17560.34676556199323055No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16580.3274130420186653No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15240.3009514330738516No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14240.2812039637120503No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13270.26204891843110306No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12990.2565196270097987No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12920.2551373041544726No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC12740.25158275966934834No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12020.23736458172885144No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11740.23183529030754707No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11060.21840701114152222No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10940.21603731481810604No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10930.21583984012448804No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10040.1982645923924849No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9920.19589489606906874No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9690.19135297811585444No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9520.18799590832434823No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9470.18700853485625815No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9380.18523126261369605No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8760.17298783160937928No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8750.17279035691576122No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8560.16903833773701898No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8060.15916460305611835No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8000.1579797548944103No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7710.15225298877948792No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7370.14553884919647547No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7320.14455147572838542No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7020.13862723491984502No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6960.13744238675813694No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6620.13072824717512452No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6600.1303332977878885No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6590.13013582309427046No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6560.12954339901341644No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6470.1277661267708543No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6320.12480400636658412No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6320.12480400636658412No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA6270.12381663289849407No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6220.122829259430404No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6160.1216444112686959No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6110.12065703780060584No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC6010.11868229086442572No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5970.11789239208995367No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5830.11512774637930148No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5780.11414037291121143No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5780.11414037291121143No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5690.11236310066864931No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5680.1121656259750313No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5670.1119681512814133No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5500.10861108148990707No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5290.10446411292392879No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5270.10406916353669278No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5260.10387168884307477No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5200.10268684068136667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)