FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007960596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533190
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG28610.5365817063335772No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT24030.45068362122320377No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG21760.4081096794763593No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG21080.39535625199272306No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC20600.3863538325925092No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18550.3479059997374294No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA17550.32915095932031735No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA16750.31414692698662766No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16720.3135842757741143No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT15110.2833886607025638No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14390.2698850316022431No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG13960.2618203642228849No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12850.24100226935989047No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG12270.23012434591796546No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC11020.20668054539657532No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10890.20424239014235077No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10490.19674037397550592No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC10140.1901761098295167No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10140.1901761098295167No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC9240.17329657345411578No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG9130.17123351900823347No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8920.16729496052063994No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8180.15341623061197696No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT8060.1511656257619235No Hit
GGACTGGCCACGGTGGCCACCATCGTAGCGTTCCCAGCCGCTCG7720.1447889120201054No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7720.1447889120201054No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT7570.14197565595753858No Hit
CGGGAAAGACAGATCTTCGTCAGAAGAAGAGCTGGATGCCGCAG7450.13972505110748512No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7360.13803709746994505No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG7350.13784954706577393No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7260.13616159342823383No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7250.1359740430240627No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC7110.133348337365667No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT6780.12715917402802002No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6750.12659652281550665No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6640.12453346836962434No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6630.1243459179654532No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA6590.12359571634876873No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT6590.12359571634876873No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6540.12265796432791314No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6480.1215326619028864No Hit
CGGACCAGGATCCGGACCGTCGTCGCCCATCTGATCCGGTTCCG6240.1170314522027795No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6200.11628125058609502No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5910.1108422888651325No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5840.10952943603593467No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG5710.10709128078171008No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5630.10559087754834111No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5630.10559087754834111No Hit
ACGCAGAATGGAGGTCGGGGTGGAACGGTCAGACTGCGGTGGAG5590.10484067593165665No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC5340.1001519158273786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)