FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007960599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554252
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA51430.927917265070762No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT34170.6165065710182372No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG28630.5165520377012622No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24960.45033666996239974No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23710.4277837517952123No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23460.42327316816177474No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG21140.3814149520434748No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20500.3698678579418748No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC17610.31772551113933734No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16610.29968317660558735No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15140.27316094484097486No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14610.2635985075380874No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13590.2451953263136624No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG13460.24284982282427486No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12130.21885351789438737No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC11920.21506462764229992No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA11000.19846567987124988No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10310.18601646904296243No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA10000.1804233453374999No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9770.17627360839473744No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC9630.1737476815600124No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9130.16472651429313742No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9020.1627418574944249No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8980.1620201641130749No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8550.15426196026356243No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8350.1506534933568124No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8110.14632333306871242No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8040.1450603696513499No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA7990.14415825292466242No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7950.14343655954331241No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC7890.14235401947128742No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA7780.14036936267257494No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7710.13910639925521243No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7550.13621962572981244No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC7520.13567835569379993No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG7340.13243073547772494No Hit
CAGGCGCCGTACCCGTCTGAGGACTCCCCGA7220.13026565533367493No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7200.12990480864299994No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7150.12900269191631245No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6930.12503337831888744No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6760.12196618144814993No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6580.11871856123207493No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6520.11763602116004995No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6500.11727517446937495No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6360.11474924763464996No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6350.11456882428931243No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG6330.11420797759863743No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6110.11023866400121243No Hit
GCAGTGGCGAGCAAATATGGTCTGAGCCTGG5800.10464554029574996No Hit
ATGAACCCGTCCACCCACGTTTCTAGCAACG5720.10320215353304994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)