FastQCFastQC Report
Wed 8 May 2024
EGAF00007960601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539400
Total Bases16.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49540.9184278828327772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44480.8246199480904709No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36480.6763070077864294No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG27750.514460511679644No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24470.453652206154987No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23260.43121987393400074No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21980.4074898034853541No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG20070.3720800889877642No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18480.34260289210233597No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC16490.3057100482017056No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16200.3003337041156841No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14270.26455320726733406No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14100.26140155728587317No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12540.23248053392658508No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG12420.23025583982202447No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC11890.22043010752688172No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11500.2131998516870597No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA11250.20856507230255839No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10710.19855394883203561No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC9820.18205413422321098No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA9430.17482387838338895No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8900.1649981460882462No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8700.16129032258064516No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8570.15888023730070447No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8490.15739710789766406No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC7970.14775676677790137No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7970.14775676677790137No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA7870.14590285502410086No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7620.14126807563959953No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7620.14126807563959953No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC7450.13811642565813867No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC7370.13663329625509826No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA7330.13589173155357806No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7310.13552094920281796No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7170.13292547274749722No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6980.12940304041527623No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6980.12940304041527623No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6790.12588060808305523No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6700.12421208750463479No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6590.1221727845754542No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6580.12198739340007415No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6400.11865035224323323No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6380.11827956989247312No Hit
CAGGCGCCGTACCCGTCTGAGGACTCCCCGA6380.11827956989247312No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6360.11790878754171302No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5930.10993696700037078No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5780.10715609936967001No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5690.10548757879124952No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5410.10029662588060809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)