FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007960607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509506
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA32620.6402279855389338No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32360.6351250034346995No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT28120.5519071414271863No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG23130.45396913873438194No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20630.4049020031167445No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19690.38645276012451274No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19660.3858639544971011No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17240.33836696721922804No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17190.33738562450687526No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14600.2865520720070029No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13160.25828940189124366No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC12670.24867224331018672No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12590.24710209497042232No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12330.24199911286618805No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12180.23905508472912979No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT12090.23728866784689484No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11250.22080211027936864No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11090.21766181359983983No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10770.21138122024078224No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10630.20863346064619454No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9800.1923431716211389No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9680.1899879491114923No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8960.17585661405361272No Hit
GAAGAGCTGTCCAACGAAGTTCTGCCACCGC8700.17075363194937843No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8700.17075363194937843No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8540.16761333526984962No Hit
GGCGGCGGTGGCGCAAGCGGTGGCGGCGGTG8230.16152901045326257No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8190.16074393628338038No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA7670.15053797207491176No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7630.14975289790502957No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7600.14916409227761793No Hit
CTGCGTTTCAAAACCGGCCGTAACGCTGCGT7350.14425737871585417No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7320.14366857308844252No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7210.14150961912126647No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6980.13699544264444385No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6550.12855589531821018No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG6210.1218827648742115No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC6000.11776112548232995No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5530.10853650398621409No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5510.108143966901273No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5460.10716262418892025No Hit
GGTGAACTGGCTGCATCTGCGGCATCCCTGA5460.10716262418892025No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5340.10480740167927365No Hit
CGTTCTGAAGAAGACAAACGTTTCGAATCCC5320.10441486459433255No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5280.10362979042445035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)