FastQCFastQC Report
Wed 8 May 2024
EGAF00007960845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450672
Total Bases14.8 Mbp
Sequences flagged as poor quality0
Sequence length33
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACTGG52411.1629300244967515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22460.4983668832321511No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTCGT22030.48882557602868604No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTGAC19120.42425533425639933No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAACTG18060.40073490254553207No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCGAT17060.37854581602584586No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTGAG16030.35569105691056907No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCTAC12880.2857954343735577No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTCGT12710.28202328966521106No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGTTC12580.27913870841765187No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCAGC12400.2751446728441083No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCCGT11740.2604998757411155No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAACTG11040.24496751517733517No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACGTT10910.24208293392977598No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCGGT10570.23453864451308268No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGATG10550.23409486278268896No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTAAC9900.21967195654489297No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACCAC9640.21390279404977455No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCGAA9200.20413959598111264No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAGAA8910.19770476089040365No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATGTA8660.19215748926048212No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGGAC8590.1906042532041041No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACACT8380.18594454503497No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTATG7980.17706891042709555No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCGGT7610.16885894841481167No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAGAA7040.15621116909859056No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTCGC6980.15487982390740937No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAACAG6670.14800120708630668No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTAAA6370.14134448113040082No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGGGC6360.14112259026520396No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAGCC6060.1344658643092981No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGCTG5970.13246884652252636No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTGAA5960.1322469556573295No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATCAC5860.13002804700536089No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACCTG5770.12803102921858914No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCTTC5580.12381510277984875No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTGGT5470.12137430326268328No Hit
CAGGGCCGTTATACTACCAACGACGGTTACGTT5340.11848972201512409No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTTCC5280.1171583768239429No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTCGT5130.11383001384598998No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTGGC5120.11360812298079313No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAACAG5080.11272055952000568No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTGCT5070.11249866865480883No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATCAC4990.11072354173323393No Hit
GCGGCTGGTCTGGCAATTTCTCAGCGCATGACC4890.10850463308126532No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATGTT4820.10695139702488728No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGTTC4810.10672950615969043No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCAGC4550.10096034366457202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)