FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007961103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459830
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56461.2278450731792183No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT23330.5073614161755432No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23230.5051866994323989No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20520.44625187569319097No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC19130.4160233129634865No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17150.37296392144923124No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14550.3164212861274819No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13630.29641389209055524No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13280.28880238348955045No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12990.28249570493443227No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA12970.28206076158580345No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12590.2737968379618555No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12390.2694474044755671No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12130.2637931409433921No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11850.25770393406258835No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10580.23008503142465694No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10330.22464823956679641No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC9990.21725420264010614No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC9610.20899027901615813No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9330.20290107213535435No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8890.19333231846551988No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8800.19137507339669008No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8270.1798490746580258No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8130.1768044712176239No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7760.16875801926799033No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT7190.15636213383206837No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6540.14222647500163105No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC6230.135484853097884No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA6140.1335276080290542No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6090.1324402496574821No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6040.13135289128591002No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5840.1270034577996216No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5750.1250462127307918No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5680.12352391101059088No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5640.1226540243133332No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5580.12134919426744666No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT5080.11047561055172563No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4980.10830089380858145No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG4840.10525629036817953No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4790.10416893199660744No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC4700.10221168692777766No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4640.10090685688189113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)