FastQCFastQC Report
Wed 8 May 2024
EGAF00007961107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468757
Total Bases14.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52511.120196605064031No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24130.5147656461663506No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT23720.5060191101146223No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA21340.45524653498507756No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC19240.4104472039884204No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16910.36074127959689134No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC15780.33663497291773775No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14660.31274199638618727No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14140.3016488287108246No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14100.30079550812041206No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA12700.270929287455974No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12650.26986263671795835No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12640.26964930657035524No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12540.26751600509432394No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12430.26516937347068953No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11110.23700979398707647No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC10780.2299698991161732No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10770.22975656896857005No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9770.20842355420825714No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC9770.20842355420825714No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9140.19498375490926No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9050.19306378358083187No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8760.1868772093003411No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8380.17877066369142222No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7530.16063760114515624No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7400.15786430922631556No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT7300.15573100775028426No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC6650.14186454815608088No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA6330.13503798343278076No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6330.13503798343278076No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6260.13354467239955883No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6190.1320513613663369No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6000.12799808856187747No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT5910.1260781172334493No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5880.12543812679063993No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5720.12202484442898986No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5650.12053153339576797No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5350.11413162896767408No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5200.11093167675362714No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG5140.10965169586800838No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4960.10581175321115205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)