FastQCFastQC Report
Wed 8 May 2024
EGAF00007961171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460413
Total Bases14.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54471.183068245249374No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA15240.33100716096200583No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13470.2925634158896469No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8570.18613722896616733No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8570.18613722896616733No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8550.18570283636647966No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7650.16615516938053443No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6760.14682469869443304No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6640.144218343096307No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6100.1324897429047399No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5600.12162992791254809No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5600.12162992791254809No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5370.11663441301613985No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5160.1120732907194193No Hit
ATGTCCAACATGGACATCGATGGTATTAACA4970.10794656102238642No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4630.10056188682769601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)