FastQCFastQC Report
Wed 8 May 2024
EGAF00007961227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424168
Total Bases13.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51981.2254578374606286No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19020.4484072348692028No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16690.3934761698195055No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15110.356226778069067No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13470.31756285245468774No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13430.3166198298787273No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12960.3055393146111918No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12680.29893815657946854No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9420.2220818166386903No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8770.20675769977933273No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8400.1980347409516984No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7840.18483242488825183No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7750.18271062409234076No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7340.17304464268874598No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7090.16715075158899304No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6600.1555987250334773No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6540.1541841911695366No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6400.15088361215367496No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5710.13461647271835686No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5280.12447898002678184No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5210.12282869051885102No Hit
GCTGATCGTCAGGCAAAAGCTAAAGCACTGC5210.12282869051885102No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5010.11811357763904869No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4770.11245544218328586No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4760.11221968653929576No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4720.11127666396333528No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4630.10915486316742423No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4620.10891910752343412No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4490.10585428415156259No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4430.10443975028762188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)