FastQCFastQC Report
Wed 8 May 2024
EGAF00007961231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452533
Total Bases14 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59591.3168100447923134No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA36560.8078968826582813No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT32320.7142020581924412No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG21300.4706839059250928No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17700.3911316964729644No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16730.3696967955928076No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16290.35997374777088076No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15230.3365500416544208No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10700.2364468447604926No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9790.21633781403787128No Hit
ATGTCCAACATGGACATCGATGGTATTAACA9450.20882454981183693No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9380.2072777012947122No Hit
ACTCTGACCGGTAAAGTTGAGGATAACCACG8810.1946819347981252No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8210.1814232332227705No Hit
GCAGAAGCGATGGAAGTGGCGAACAAAACCC7790.17214214212002218No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC7330.1619771375789169No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7240.1599883323426137No Hit
GCAGCCGAAGCGATGGAAGTCGCTTCCCAGG6940.15335898155493632No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6590.14562473896931272No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6310.13943734490081386No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6030.13324995083231497No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC5980.13214505903436877No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA4560.1007661319726959No Hit
TCTACCCCGGCCAGCACTGGTGGTGTTGGTC4560.1007661319726959No Hit

[OK]Adapter Content

Adapter graph