FastQCFastQC Report
Wed 8 May 2024
EGAF00007961232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478939
Total Bases21 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG23730.49547019557814254No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT18440.38501771624361353No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17680.3691493071142672No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15780.32947828429090137No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14370.30003820945882465No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA13550.2829170311876878No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC13370.27915872376231626No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12880.2689277757710272No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11340.23677336779840438No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11280.2355205986566139No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG10130.21150919010562932No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9450.19731113983200368No Hit
ATAACGCAGGTAAGAGTACACTTCGCGTTCTTCTTCGGTATAAC9430.19689355011807347No Hit
TCATTTGAAACGCTGCATCTGGACGCCCATACGTTTCTGGTAGG8790.18353067927230818No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8730.18227791013051767No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8720.18206911527355257No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8060.1682886547138571No Hit
ACCCAGCGGGATGGCCGGCAGAGAATAGCCATCCGGAAAAACGG7660.15993686043525376No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7380.15409060444023143No Hit
TTTGAAACGCTGCATCTGCACGCCCATACGTTTCTGGTATGCCT6660.13905737473874544No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6330.13216714445889768No Hit
GGAGCTCGGACCTACGTTGGTAACAGTGAACACGTCCGGACGCG6150.12840883703352618No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5960.12444173475118961No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5420.11316681247507511No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT5080.10606778733826229No Hit
CAGCAGGCCATCGACGGCCGCGGTCGCTGCATCCTGCAGGGTGC5030.10502381305343687No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT4820.10063912105717013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)