FastQCFastQC Report
Wed 8 May 2024
EGAF00007961281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470547
Total Bases14.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT107002.273949254803452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27730.5893141386513993No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24090.5119573602636931No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC18080.38423366847520013No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15330.325791047440532No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA14350.30496422248999566No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13710.2913630306855638No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13070.27776183888113193No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11330.24078359866283283No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9790.20805573088341867No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9540.20274276533481247No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8940.1899916480181576No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5910.1255985055690505No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5830.1238983565934965No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5660.1202855400204443No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5650.12007302139850004No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5550.11794783517905758No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5480.11646020482544783No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5350.1136974627401726No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5260.11178479514267438No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG5100.10838449719156641No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG4930.1047716806185142No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4900.10413412475268144No Hit

[OK]Adapter Content

Adapter graph