FastQCFastQC Report
Sun 21 May 2023
EGAF00007961285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489908
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52411.069792695771451No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA34770.7097250912416209No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29460.6013373939596822No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG25770.5260171297468096No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15700.32046833282983744No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA15700.32046833282983744No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG15460.3155694538566425No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA14780.30168929676592343No Hit
ATGTCCAACATGGACATCGATGGTATTAACA12050.24596454844583063No Hit
TGGGGCGGTCCGGGTGCAGCTCAGGACAACT11450.23371735101284322No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11130.2271855123819166No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11060.22575667268140143No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10650.2173877544355267No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10580.21595891473501147No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA10110.206365276745838No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9780.19962931815769494No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9130.1863615209386252No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8960.18289148166594546No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8770.17901320247883276No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8540.17431844346285424No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8360.17064428423295802No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7650.15615176727058958No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6920.14125101039378823No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6860.1400262906504895No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6220.12696261338863624No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6170.12594201360255394No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5850.11941017497162734No Hit
CATCGTTCCCCACTGTGTCGTGATGAAGACG5800.11838957518554505No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5710.11655249557059692No Hit
CAGCGTACCTACTCCGCTCCGGTCCAGCAGC5650.11532777582729818No Hit
ATGTCTCACGTCGTTGTGAAAAACGATCCGG5220.10655061766699055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)