FastQCFastQC Report
Wed 8 May 2024
EGAF00007961287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464427
Total Bases14.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59391.2787800881516365No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49011.0552788705221703No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA31660.6817002456790842No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG25300.5447573030853072No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15280.32900757277247017No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA15070.32448587183777No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG14900.32082544727158413No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13090.28185269159631116No Hit
ATGTCCAACATGGACATCGATGGTATTAACA11310.2435258931974239No Hit
TGGGGCGGTCCGGGTGCAGCTCAGGACAACT10300.22177866489243736No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10260.22091738852392304No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10070.21682632577348002No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9910.21338122029942275No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9700.20885951936472252No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA9390.20218462750873656No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9310.2004620747717079No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8670.1866816528754788No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8600.18517441923057876No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8370.1802220801116214No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8290.17849952737459276No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7620.1640731482019779No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6650.14318719626550566No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6280.1352203898567482No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6020.12962209346140513No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5950.12811485981650506No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5930.1276842216322479No Hit
CAGCGTACCTACTCCGCTCCGGTCCAGCAGC5390.1160569906573046No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5250.11304252336750448No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5160.11110465153834725No Hit
GGTCTGCAGGACGATGTTGCACAGTTCGTGC4800.10335316422171838No Hit
CATCGTTCCCCACTGTGTCGTGATGAAGACG4760.10249188785320407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)