FastQCFastQC Report
Wed 8 May 2024
EGAF00007961288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493052
Total Bases21.6 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG25440.5159699179802536No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA22820.4628315066159351No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA20140.40847618506770084No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15530.3149769192701784No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG15530.3149769192701784No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15390.3121374621743751No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG15130.30686418471074045No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG11850.24033976132334925No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11400.23121293494398157No Hit
AACTTCCAGGCCCGGCAGTACGGTTGCCAGGCGACGTTTACGAC11400.23121293494398157No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10840.21985510656076843No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG10780.21863819637685275No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10710.2172184678289511No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10600.21498746582510564No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9590.19450281106252484No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8400.17036742574819694No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7890.16002368918491355No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT7400.15008558934960206No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7320.14846304243771447No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6890.13974185278631868No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6760.13710521405450135No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA6500.13183193659086667No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG6440.13061502640695097No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6290.12757275094716175No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5720.11601210419996268No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5400.10952191655241232No Hit
ACCCAGAGAACGAATCCAGGTGGTACGGCCCTGAACACGCAGTA5380.10911627982444043No Hit
ACGCGCGGAGTAAGAGCCACCGGATTCACGGCCTGCGATCCACG5230.10607400436465118No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5200.10546554927269335No Hit
GTCGTGGAAGCCTTTGCTAGCTTCACGGTTACGCAGATGCGGTG5180.10505991254472144No Hit
TGCACGCGGCGCGCGAGCACCCGGCGGCAGGTGACGACGTTTAC5140.10424863908877766No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC5130.10404582072479171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)