FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007961335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468555
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63661.358645196401703No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA33990.7254217754585908No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16440.35086596023945965No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15550.3318713918323356No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13970.2981506973567671No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG11220.23945961519992315No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT10540.22494691124841265No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10540.22494691124841265No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10090.21534291598638367No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10000.21342211693397786No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9910.21150131788157206No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9770.20851340824449638No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA9210.19656176969619363No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8800.1878114629019005No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8620.18396986479708893No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8400.1792745782245414No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8160.17415244741812594No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8130.173512181067324No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7960.16988400507944637No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7840.16732293967623865No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7810.1666826733254367No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7050.15046259243845442No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6970.1487552155029826No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6800.14512703951510494No Hit
GCGGCTGGTCTGGCAATTTCTCAGCGCATGA6730.1436330846965671No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6400.13659015483774584No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC6280.1340290894345381No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6010.1282666922773207No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5740.1225042951201033No Hit
AAACGTATCAACAGCGCTGCCGACGACGCAG5610.11972980759996157No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5570.11887611913222566No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5550.11844927489835771No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5520.11780900854755577No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5230.11161976715647043No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5140.10969896810406463No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5110.10905870175326268No Hit
GGCCTGACCATTAGCGAGAAGATGCGTAAAC5050.10777816905165882No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4990.10649763635005496No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC4980.10628421423312098No Hit
ATCAACCGTGCAGCGGACGATGCGGCTGGTC4940.10543052576538507No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT4860.10372314882991325No Hit
GGTCTGACCATTAGCGAAAAAATGCGCAAAC4830.10308288247911132No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA4720.10073523919283756No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4720.10073523919283756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)