FastQCFastQC Report
Wed 8 May 2024
EGAF00007961336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499458
Total Bases21.9 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19120.3828149714290291No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15310.306532280992596No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13480.26989256353887614No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12830.25687845624657124No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11550.23125067573249403No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC11060.22144004100444883No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG10170.20362072486575447No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10100.2022192056188909No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10000.20021703526622858No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9900.19821486491356632No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT9650.1932094390319106No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA9370.18760336204445618No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8450.16918339479996314No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG8420.16858274369416448No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8170.16357731781250878No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG8040.16097449635404779No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7890.15797124082505437No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7790.15596907047239209No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7130.14275474614482098No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG7130.14275474614482098No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7000.14015192468636004No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG6920.1385501884042302No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6770.13554693287523675No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG6640.13294411141677578No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG5970.11952957005393847No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG5790.11592566341914634No Hit
ATCGCTGCTGTTGTTGGTAGAGTTCGCGGCCTGAACGGACAGTT5790.11592566341914634No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG5750.11512479527808143No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5700.11412371010175029No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5650.11312262492541915No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG5400.10811719904376343No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5400.10811719904376343No Hit
CAGGGAGTTAGTCAGAGAGGTCAGGCCGCTAGACGCGGTCTGCA5300.10611502869110115No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG5170.1035122072326402No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5150.10311177316210773No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5050.10110960280944545No Hit
GAACTCGGTCAGGCCCATGCGGCGTTTGTTGGAGATGTCGTAAA5030.10070916873891299No Hit

[OK]Adapter Content

Adapter graph