FastQCFastQC Report
Wed 8 May 2024
EGAF00007961355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456963
Total Bases14.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57861.266185664922543No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36370.7959068896168837No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG18000.3939049769893842No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15610.3416031494891271No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA15140.3313178528677377No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9970.2181795900324534No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9150.20023502996960366No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8990.1967336523963647No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8490.18579184747999292No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7090.1551547937141519No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6950.1520910883375678No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6750.1477143663710191No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6660.14574484148607217No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6360.13917975853624912No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG6120.13392769217639064No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6000.13130165899646143No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5940.12998864240649682No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5810.12714377312824016No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5740.1256119204399481No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5690.12451773994831092No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5580.12211054286670912No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5500.12035985408008965No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5460.11948450968677989No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5150.11270059063862939No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5100.1116064101469922No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5070.1109499018520099No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5030.11007455745870016No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4950.10832386867208067No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4930.1078861964754258No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4720.10329063841054964No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4670.10219645791891248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)