FastQCFastQC Report
Wed 8 May 2024
EGAF00007961820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459477
Total Bases20.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG19100.41569001277539464No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16920.36824476524396216No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG16210.35279241398372496No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14230.3096999414551762No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14200.30904702520474364No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13010.28314801393758554No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11250.2448435939122089No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG11200.24375540016148795No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10320.22460319014879962No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10160.22112097014649265No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG10020.21807402764447403No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9990.2174211113940415No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG9460.20588625763639962No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG8420.18325182762140435No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8290.18042252386952992No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8000.17411100011534855No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7940.17280516761448345No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7800.16975822511246483No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG7730.1682347538614555No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7620.16584072760986948No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7580.16497017260929275No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7360.16018212010612065No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7180.15626462260352533No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6570.14298865884472997No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT6550.14255338134444162No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6390.13907116134213465No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6280.1366771350905486No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6030.13123616633694396No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5930.12905977883550213No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5470.11904839632886956No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5310.11556617632656259No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5260.11447798257584166No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5000.10881937507209284No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG5000.10881937507209284No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT4890.1064253488205068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)