FastQCFastQC Report
Wed 8 May 2024
EGAF00007961927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007961927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448151
Total Bases13.8 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58161.2977768653868897No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT29900.6671858369165751No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA22200.495368748479865No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16140.3601464684894154No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15010.3349317529136385No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10020.22358535404361476No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9300.20751934057940294No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9250.20640364519994378No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9090.20283341998567445No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8540.19056077081162376No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8350.18632112836967896No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7970.1778418434857894No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6780.1512882934546615No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6070.13544541906634147No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6020.1343297236868823No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5820.1298669421690457No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5700.12718927325834373No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5690.1269661341824519No Hit
CGTCGCGGTCTGGATCTGCTGTTCTGGGAAC5170.11536290223607669No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4790.1068836173521871No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA4540.10130514045489133No Hit
GGCAAAATCCCGGGCAACCTGATCACCAACG4530.10108200137899949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)