FastQCFastQC Report
Wed 8 May 2024
EGAF00007962077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499009
Total Bases15.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA69451.3917584652781814No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35610.7136143837085103No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA34340.688163940930925No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA30270.6066022857303175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25580.512616004921755No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25150.5039989258710764No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG20760.4160245606792663No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19810.3969868278928837No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15320.30700849082882276No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12290.24628814309962344No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12250.24548655435072314No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12230.24508575997627297No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11190.22424445250486466No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9370.18777216442990005No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9260.1855677953704242No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8500.1703376091413181No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8070.16172053009063964No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7960.15951616103116378No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7930.15891496946948852No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7870.15771258634613805No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7670.15370464260163644No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7560.15150027354216056No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7380.14789312417210912No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA7370.14769272698488403No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG7320.14669074104875862No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6580.13186134919410272No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5940.11903592921169757No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5880.11783354608834712No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5610.11242282203326995No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5590.11202202765881977No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5490.11001805578656897No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5280.1058097148548423No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5230.1048077289187169No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5190.10400614016981657No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5170.10360534579536643No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5170.10360534579536643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)