FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007962079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465803
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA63851.3707511544580004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61281.3155776154296988No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32850.7052337576185641No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA31010.665732079870675No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA28990.6223661075604924No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23490.5042904403793019No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19890.42700454913343194No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18150.38964970169792806No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13850.2973359982653611No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11680.25074978048660057No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10910.23421918708123393No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10850.23293108889380276No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10210.21919137489453694No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8300.17818691592797814No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8300.17818691592797814No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7940.17045832680339115No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7800.16745276436605172No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7720.1657353001161435No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA7220.15500114855421715No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7130.15306900127307038No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7080.15199558611687772No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6790.14576977821096043No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6790.14576977821096043No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6730.14448168002352926No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6580.14126143455495135No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6100.130956649055502No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5560.11936376536862149No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5440.11678756899375917No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5170.11099112715031892No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5010.10755619865050246No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC4950.1062681004630713No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4950.1062681004630713No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4930.10583873440059424No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4920.10562405136935571No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4890.10498000227564014No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4860.10433595318192455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)