FastQCFastQC Report
Wed 8 May 2024
EGAF00007962080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504274
Total Bases22.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT34410.6823671258085882No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC30280.6004672063203734No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26710.5296723606610692No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA23810.4721639426184971No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC23400.4640334421366162No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA19940.39541995026513366No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA17190.34088610556959115No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16390.3250217143854333No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG14470.28694717554345456No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13790.2734624430369204No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12520.24827772203206985No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12150.24094044110939689No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11600.23003367217028836No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG11100.22011842768018974No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8420.16697271721326104No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG8390.16637780254385512No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7710.152893070037321No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA7520.1491252771310835No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC7280.14436595977583616No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7170.14218460598801444No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7130.14139138642880655No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6870.13623545929395528No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6740.13365749572652963No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6700.13286427616732174No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6700.13286427616732174No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT5610.1112490431789067No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5570.11045582361969883No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5440.10787786005227316No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5300.10510159159504555No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5160.10232532313781795No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5100.10113549379900609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)