FastQCFastQC Report
Wed 8 May 2024
EGAF00007962081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504274
Total Bases15.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA70521.3984460828835117No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC36310.720045054870963No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA34030.6748315399961132No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31270.6200993904107688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27270.5407774344899797No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25770.5110317010196838No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22250.44122837980938934No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19540.38748775467305474No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14950.29646581025394925No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12600.24986416115048565No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12590.24966585626068366No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12300.2439150144564265No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11110.22031673256999168No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9290.18422524262603268No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9180.182043888838211No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8240.16340322919682554No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8160.16181679007840974No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8150.16161848518860777No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA7870.15606594827415254No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG7840.15547103360474662No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7630.1513066309189052No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7610.15091002113930124No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7430.14734053312286574No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7340.14555578911464798No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7280.14436595977583616No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6860.1360371544041533No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5960.11818971432197575No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5930.11759479965256985No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5740.11382700674633237No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5640.11184395784831261No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5560.11025751872989684No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5460.10827446983187712No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5210.1033168475868278No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5210.1033168475868278No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5200.10311854269702582No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5140.10192871335821399No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5060.10034227423979819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)